| 质粒名称: | pAAV-CAG-mBaoJin(codon-optimized for mouse) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000464 |
| 产品用途: | to express mBaoJin(codon-optimized for mouse) in mouse |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mBaoJin(mouse) |
| 物种来源: | human |
| 密码子优化: | 老鼠表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Leslov G.D., Subach O.M., Zhang W., Kuzmicheva T.P.P., Vlaskina A.V.V., Samygina V.R.R., Chen L., Ye X., Nikolaeva A.Y.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S.S., Drobizhev M., Mishin A.S.S., Piatkevich K.D.
Nat. Methods 2024 214 21, 657–665 (2024)
|
买家导航
pAAV-CAG-mBaoJin(codon-optimized for mouse)载体质粒基本信息
pAAV-CAG-mBaoJin(codon-optimized for mouse)质粒图谱载体图谱和pAAV-CAG-mBaoJin(codon-optimized for mouse)载体序列质粒序列多克隆位点信息
载体图谱.png)
pAAV-CAG-mBaoJin(codon-optimized for mouse)质粒载体简介
pAAV-CAG-mBaoJin(codon-optimized for mouse)质粒序列载体序列
LOCUS pAAV-CAG-mBaoJin(codon-optimized for mouse) 5284 bp DNA circular SYN 27-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5284)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..5284
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 281..558
/label=chicken beta-actin promoter
misc_feature 982..991
/label=Kozak sequence
CDS 988..1689
/codon_start=1
/label=mBaoJin(codon-optimized for mouse)
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
primer_bind complement(1000..1018)
/label=mBaoJin-R
primer_bind complement(1670..1716)
/label=0324-CLEM-mBJ-R1
misc_feature 1717..2305
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2188..2199)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2348..2469
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 2359..2378
/label=58-SV40poly(A)-Rseq
repeat_region 2631..2688
/label=AAV2 ITR
rep_origin complement(2848..3436)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3607..4467)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4468..4572)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4598..5053)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 4977..4996
/label=121-WKG-450-F1
repeat_region 5125..5254
/label=AAV2 ITR
ORIGIN
1 tatctacgta gccatgctct aggaagagta ccattgacgt caataatgac gtatgttccc
61 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
121 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
181 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
241 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
301 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
361 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
421 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
481 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
541 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
601 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
661 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
721 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
781 ggctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
841 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
901 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
961 agaattggat ccggtaccta agccaccatg gtcagcaaag gagaggaaga gaatatggct
1021 tccaccccct ttaagtttca actcaaggga acaatcaacg gcaaatcatt cacagtggaa
1081 ggtgagggag agggaaactc ccacgaaggc tctcataaag gaaagtacgt ttgtacctct
1141 ggaaaacttc ctatgagctg ggctgcactc gggactacat tcgggtacgg gatgaaatac
1201 tatactaaat acccaagtgg actgaaaaat tggtttaggg aagttatgcc tggagggttc
1261 acctatgaca gacacataca gtataaaggc gacggcagca tacatgctaa gcaccagcat
1321 tttatgaaaa acggcaccta tcataatatt gtggagttta ccggtcaaga ttttaaagag
1381 aattcacccg tactcaccgg agacatgaac gtatcactgc caaatgaagt ccctcaaatt
1441 cctagggatg acggtgtaga gtgtccagtg actctcctct accctcttct gagtgataag
1501 tctaagtacg tcgaagccca ccaatatacc atctgcaaac ccctgcacaa tcagccagct
1561 ccagatgttc cctaccactg gatccgcaaa caatataccc aatcaaaaga cgacgccgaa
1621 gaacgggatc atatctgcca atcagaaact cttgaagcac atctcaaagg gatggacgaa
1681 ctttacaagt aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
1741 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
1801 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
1861 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
1921 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
1981 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
2041 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
2101 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
2161 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
2221 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
2281 atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca
2341 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
2401 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
2461 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
2521 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct
2581 agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct
2641 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgact cgctgcgctc
2701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
2761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
2821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
2881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
2941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
3001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
3061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
3121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
3181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
3241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
3301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
3361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
3421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
3481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
3541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
3601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
3661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
3721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
3781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
3841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
3901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
3961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
4021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
4081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
4141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
4201 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
4261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
4321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
4381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
4441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
4501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
4561 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
4621 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
4681 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
4741 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
4801 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
4861 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
4921 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
4981 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
5041 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
5101 cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca
5161 aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
5221 gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa cccgccatgc
5281 tact
//
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